Protocols

This Standard Operating Procedure (SOP) provides instructions for preparing and examining fresh stool samples collected from participants of the ALIVE trial for the diagnosis of Strongyloides stercoralis infection by a modified Baermann technique. This protocol is applicable to all fresh stool samples collected and received at the laboratory for testing in the context of the ALIVE trial.

File: SOP_006_v02   |  Date: 20 Dec 2021

This Standard Operating Procedure (SOP) describes the procedures for Soil Transmitted Helminths eggs screening and quantitation by the Kato-Katz technique from stool samples for participants in the ALIVE Trial. This protocol is applicable to all fresh stool samples collected in the context of the ALIVE trial and received in the Parasitology Laboratory for testing.

File: SOP_005_v02   |  Date: 24 Feb 2022

This Standard Operating Procedure (SOP) describes the method for extracting genomic DNA from either ethanol preserved or unpreserved stool samples. The SOP includes two different steps. The first step describes how to remove ethanol from the preserved samples. The second step describes the process of DNA extraction using the QIAamp DNA Mini Kit, including a bead beating phase which is recommended to facilitate the DNA extraction by mechanical disruption of the helminth’s eggs.

File: SOP_007_v02   |  Date: 20 Dec 2021

This Standard Operating Procedure (SOP) describes the steps required for (i) ordering, (ii) receiving, (iii) determining the concentration of the working solution to be added to each sample, and (iv) aliquoting of the stock solution of PhHV when used as DNA extraction internal control in the context of the ALIVE trial.

File: SOP_014_v02   |  Date: 20 Dec 2021